Increased Anticancer Activity of Organic Kimchi with Starters Demonstrated in HT-29 Cancer Cells

This study aims to investigate the effects of kimchi made with organic ingredients and lactic acid bacteria (LAB) as starters (Leuconostoc mesenteroides + Lactiplantibacillus plantarum) on HT-29 human colon carcinoma cells. Four types (standard (SK), commercial (CK), anticancer (AK), (OAK)) were evaluated. The results show that, among different studied, OAK presents high DPPH free-radical scavenging activity total phenol flavonoid contents, MTT assay shows that growth inhibition rate against cancer cells is highest. In addition, quantitative reverse transcription polymerase chain reaction (RT-qPCR) compared SK CK, AK can effectively down-regulate mRNA expression anti-apoptotic gene Bcl-2 up-regulate cell cycle arrest genes p21 p53; pro-apoptotic Bim, Bak, Bad; for caspases 3 9. Subsequently, a Western blot test confirmed decreased expressions p53, Bax, 9 increased in OAK. abovementioned indicate prepared present best antioxidant inhibit proliferation by promoting apoptosis arrest.